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For any AUSAs here who transferred between offices, do you have to re-do your background investigation, or does it transfer over? I was favorably adjudicated last summer, and I was under the impression that I won't be re-investigated for five years, but I wasn't sure if transferring offices changes that.

No, it transfers over. I got hired at my current office about 4 years after starting at my previous one and one of the factors in my favor was that I was a current AUSA and so they didn't have to wait for me to do the background check and just had to change my office in the various systems, not get me established all over again, as there were various reasons why they needed to get the person on the job quickly.

(I feel obligated to point out that you don't transfer between offices just by asking - you wait for an opening and apply when it opens up like the rest of the world. They will transfer you over administratively, but it's not transferring in the sense that you can request it outside of the usual employment process. I'm sure you know that already, but I say it just b/c I do run into people who think you can just put in for a transfer. The exception is that there is such a thing as a hardship transfer, but IME it's limited to spouses of active military who get stationed elsewhere, and even then, the office they're transferring to gets to vet them before signing off.)